Plasmid

Part:BBa_K4876008

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


gRNA-lacZ

gRNA-lacZ

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 328
    Illegal XbaI site found at 334
    Illegal SpeI site found at 219
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 328
    Illegal NheI site found at 196
    Illegal SpeI site found at 219
    Illegal PstI site found at 346
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 328
    Illegal BglII site found at 358
    Illegal BamHI site found at 184
    Illegal XhoI site found at 382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 328
    Illegal XbaI site found at 334
    Illegal SpeI site found at 219
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 328
    Illegal XbaI site found at 334
    Illegal SpeI site found at 219
    Illegal PstI site found at 346
    Illegal NgoMIV site found at 1153
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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